Journal: Applied and Environmental Microbiology

Article Title: A Counterselectable Sucrose Sensitivity Marker Permits Efficient and Flexible Mutagenesis in Streptococcus agalactiae

doi: 10.1128/AEM.03009-18

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Strains E. coli DH5α Chemically competent cloning strain New England Biolabs item no. C2987H B. subtilis 168 Legacy strain derived from B. subtilis Marburg 39 GBS CNCTC 10/84 Serotype V, sequence type 26 40 GBS A909 Serotype Ia, sequence type 7 41 GBS AR1598 Serotype V, sequence type 26 10/84 mutant with barcoded premature stop codon in cylE gene This study Plasmids pDC123 Shuttle vector with the chloramphenicol acetyltransferase gene used as PCR template 42 pHY304 Broad-host-range, temperature-sensitive mutagenesis plasmid; pWV01 derivative; erythromycin resistant 12 pMBsacB pHY304 with p23- sacB cassette cloned into AvaII site, for sucrose-counterselectable GBS mutagenesis This study pORI23 Shuttle vector with ori ColE1 and Lactococcus -derived Gram-positive origin of replication; erythromycin resistant; p23 promoter adjacent to BamHI restriction site 43 pSacB23 pORI23 with sacB cloned into BamHI site for expression off p23 promoter This study Open in a separate window Strains and plasmids used in this study Cloning technique.

Techniques: Plasmid Preparation, Cloning, Derivative Assay, Sequencing, Mutagenesis, Clone Assay, Expressing

Journal: Microbial Cell Factories

Article Title: Maintenance metabolism and carbon fluxes in Bacillus species

doi: 10.1186/1475-2859-7-19

Figure Lengend Snippet: Bacterial species used during this study

Article Snippet: B. subtilis spo0A , , trp+ spo0A- , DSM Nutritional Products Inc..

Techniques:

Journal: Microbial Cell Factories

Article Title: Maintenance metabolism and carbon fluxes in Bacillus species

doi: 10.1186/1475-2859-7-19

Figure Lengend Snippet: Relative distribution of absolute fluxes in glucose batch cultures of A) B. subtilis wild type (top), spo0A (middle-top), sigE (middle-bottom) mutants and natto (bottom) and B) B. licheniformis T218a (top), B. licheniformis T380B (middle-top), B. amyloliquefaciens (middle-bottom) and B. pumilus (bottom) . Fluxes were determined by 13 C-constrained flux analysis from two separate experiments with 100% [1- 13 C]glucose and with a mixture of 20% [U- 13 C] and 80% unlabeled glucose (Additional file ) along with the physiological data given in Table 2. Generally, the 95% confidence intervals were between 10 and 15% of the values shown for the major fluxes. Larger confidence intervals were estimated for reactions with low fluxes. To facilitate cross species comparison, fluxes are normalized to the glucose consumption rates given in Table 2. Arrowheads indicate flux direction.

Article Snippet: B. subtilis spo0A , , trp+ spo0A- , DSM Nutritional Products Inc..

Techniques: Comparison

Journal: Microbial Cell Factories

Article Title: Maintenance metabolism and carbon fluxes in Bacillus species

doi: 10.1186/1475-2859-7-19

Figure Lengend Snippet: Physiological data of glucose minimal medium batch cultures

Article Snippet: B. subtilis spo0A , , trp+ spo0A- , DSM Nutritional Products Inc..

Techniques:

Journal: Microbial Cell Factories

Article Title: Maintenance metabolism and carbon fluxes in Bacillus species

doi: 10.1186/1475-2859-7-19

Figure Lengend Snippet: Metabolic flux ratios in glucose-limited chemostat cultures at different dilution rates of A1) B. subtilis wild type, A2) spo0A and A3) sigE mutant, B1) B. licheniformis T218a (filled symbols) and T380B (open symbols) and B2) B. subtilis natto (open symbols) and B. pumilus (filled symbols) . The fraction of serine derived through glycolysis and the fraction of PEP originating from oxaloacetate were obtained from 100% [1- 13 C]glucose. All other ratios were obtained from experiments with 20% [U- 13 C]glucose and 80% natural glucose. The experimental error was estimated from redundant mass distribution [20].

Article Snippet: B. subtilis spo0A , , trp+ spo0A- , DSM Nutritional Products Inc..

Techniques: Mutagenesis, Derivative Assay

Journal: Microbial Cell Factories

Article Title: Maintenance metabolism and carbon fluxes in Bacillus species

doi: 10.1186/1475-2859-7-19

Figure Lengend Snippet: Specific glucose consumption rate and biomass concentration as a function of dilution rate in glucose-limited chemostat cultures of B. subtilis wild type 168 (A), and its spo0A (B) and sigE (C) mutants . Trend lines in the left column represent the weighed least square regression. Trend lines in the right column were drawn by hand.

Article Snippet: B. subtilis spo0A , , trp+ spo0A- , DSM Nutritional Products Inc..

Techniques: Concentration Assay

Journal: Microbial Cell Factories

Article Title: Maintenance metabolism and carbon fluxes in Bacillus species

doi: 10.1186/1475-2859-7-19

Figure Lengend Snippet: Maintenance energy coefficients in different bacilli.

Article Snippet: B. subtilis spo0A , , trp+ spo0A- , DSM Nutritional Products Inc..

Techniques:

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Enhancement of nematicidal potential through cloning and expression of chitinase gene from Bacillus subtilis subsp. Subtilis BTN7A strain

doi: 10.1016/j.jgeb.2018.05.006

Figure Lengend Snippet: Inhibition effect of cultural supernatant of E. coli transformed with chitinase gene on egg hatching of M. javanica under laboratory conditions.

Article Snippet: Greenhouse trial was conducted to evaluate the nematicidal efficiency of the supernatants of the transformed E. coli (NRC-CHI-4) and E. coli (T-CHI-NRC-6) and B. subtilis against M. javanica .

Techniques: Inhibition, Transformation Assay, Control

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Enhancement of nematicidal potential through cloning and expression of chitinase gene from Bacillus subtilis subsp. Subtilis BTN7A strain

doi: 10.1016/j.jgeb.2018.05.006

Figure Lengend Snippet: Effect of cultural supernatant of transformed chitinase - E. coli on reproduction of M. javanica associated with eggplant.

Article Snippet: Greenhouse trial was conducted to evaluate the nematicidal efficiency of the supernatants of the transformed E. coli (NRC-CHI-4) and E. coli (T-CHI-NRC-6) and B. subtilis against M. javanica .

Techniques: Transformation Assay, Control